Becoming the Bimboy (Bimbo Transformation Story, Gay Alpha Male M/M Romance)

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P. Kinnari, E. Mäkilä, T. Heikkilä, J. Salonen, J. Hirvonen and H. A. Santos, Int. J. Pharm., 2011, 414, 148–156 CrossRef CAS PubMed. In American politics, the word was used in the 1990s during Bill Clinton's sexual misconduct allegations, leading to the invention of the term " Bimbo eruptions" to refer to political sex scandals. [14] The expression was also used in a 2014 report [15] in which Colin Powell explained his reluctance to vote for Hillary Clinton in light of her husband's continued affairs with "bimbos". I do hope this video gets at least a bit of fanart. I do enjoy the bimbo look! Language: English Words: 3,113 Chapters: 1/1 Collections: 1 Comments: 2 Kudos: 111 Bookmarks: 12 Hits: 10,530 D. Liu, L. M. Bimbo, E. Mäkilä, F. Villanova, M. Kaasalainen, B. Herranz-Blanco, C. M. Caramella, V. P. Lehto, J. Salonen, K. H. Herzig, J. Hirvonen and H. A. Santos, J. Controlled Release, 2013, 170, 268–278 CrossRef CAS PubMed. H. A. Santos, L. M. Bimbo, B. Herranz, M.-A. Shahbazi, J. Hirvonen and J. Salonen, J. Mater. Res., 2013, 28, 152–164 CrossRef CAS.

L. M. Bimbo, M. Sarparanta, E. Mäkilä, T. Laaksonen, P. Laaksonen, J. Salonen, M. B. Linder, J. Hirvonen, A. J. Airaksinen and H. A. Santos, Nanoscale, 2012, 4, 3184–3192 RSC. H. A. Santos, J. Riikonen, J. Salonen, E. Mäkilä, T. Heikkilä, T. Laaksonen, L. Peltonen, V. P. Lehto and J. Hirvonen, Acta Biomater., 2010, 6, 2721–2731 CrossRef CAS PubMed. B. Guan, S. Ciampi, G. Le Saux, K. Gaus, P. J. Reece and J. J. Gooding, Langmuir, 2011, 27, 328–334 CrossRef CAS PubMed. I’m trying to bring a new definition to the word ‘bimbo’, to reclaim the word in a good way,” she says. “The way it is being used today is to empower someone to be in love with themself, almost obsessed, not having to prove their smarts or who they are. Maybe you can be ditsy and bubbly and have fun and not have to prove anything to anyone. It’s getting used to being underestimated and using it to your advantage when, despite your persona, you know your own wisdom. The word doesn’t hurt. Focus on the message.”World Cancer Report 2014, IARC, http://www.iarc.fr/en/publications/books/wcr/index.php, accessed April 2014. J.-H. Park, L. Gu, G. Von Maltzahn, E. Ruoslahti, S. N. Bhatia and M. J. Sailor, Nat. Mater., 2009, 8, 331–336 CrossRef CAS PubMed. The TEM images elucidated that the association and internalization of the bare UnTHCPSi nanoparticles with both cell lines was negligible, which can be possibly explained by the negative charge, as well as by the deficient colloidal stability of these nanoparticles. In contrast, when functionalized with HA +, and despite the less pronounced negative ζ-potential, the nanoparticles were massively associated with the cell membrane and further internalized by both MDA-MB-231 and MCF-7 cells, after which they seemed to be mainly enclosed in the endosomes of the cells. Although the aforementioned improved colloidal stability of UnTHCPSi–HA + nanoparticles favors their internalization by the cancer cells, the HA +-mediated targeting of CD44 receptor might be a driving force for the enhanced interaction and internalization of the nanoparticles by the breast cancer cells. These results represent clear evidence of the enhanced cellular association of UnTHCPSi–HA + when compared with bare UnTHCPSi nanoparticles. Flow cytometric analysis of CD44 expression and cellular association The expression of CD44 receptor in both MDA-MB-231 and MCF-7 breast cancer cells was evaluated by flow cytometry, after staining the cells with PE-CF594-labeled anti-human CD44 antibody (ESI, Fig. S2 †). When compared with the respective negative controls, both the cell lines studied were shown to express the CD44 receptor, as elucidated by the increase in the mean fluorescence intensity of the stained cells. However, the incubation of MCF-7 cancer cells with up to 4-fold increased anti-human CD44 antibody concentration was not reflected by the intensification of the mean fluorescence intensity, which experienced an approximately 9-fold increase in comparison with unstained cells regardless of the antibody concentration, thus indicating the saturation of the receptors promptly available for interacting with the complementary antibody. In contrast, in the case of MDA-MB-231 cancer cells, the successive increase of the anti-human CD44 antibody concentration resulted in the consecutive intensification of the fluorescence signal up to approximately 670-fold compared to the controls, suggesting significantly higher levels of CD44 expression in MDA-MB-231 than in MCF-7 breast cancer cells. 50 The size and morphology of the UnTHCPSi and UnTHCPSi–HA + nanoparticles were analyzed by transmission electron microscopy (TEM). The TEM pictures were captured using a Jeol JEM-1400 microscope (Jeol Ltd., Tokyo, Japan) at an 80 kV voltage. The nanoparticle suspensions were centrifuged, re-dispersed in ethanol at a concentration of 10 μg mL −1, and dropped on a carbon-coated copper TEM grid, followed by drying for 48 h at room temperature. Stability studies in human plasma For performing these experiments, 300 μg of UnTHCPSi and UnTHCPSi–HA + were dispersed in 200 μL of 1× PBS (pH 7.4), exposed to 1500 μL of human plasma, and left under stirring at 800 rpm and 37 °C for 2 h. Sample aliquots of 200 μL were withdrawn at pre-determined time points (1, 5, 10, 15, 30, 60, 90, and 120 min), and the corresponding size, ζ-potential, and PdI were subsequently determined by DLS and ELS. The results are shown as the average of at least three independent measurements. Anonymous human plasma donors were obtained from the Finnish Red Cross Blood Service, with the permission from the respective institutional ethical committee. Cell lines and culturing conditions For the in vitro studies, MCF-7 and MDA-MB-231 breast cancer cells were cultured according to the protocols described in detail in the ESI. † In vitro cytotoxicity studies The in vitro cytotoxicity of both UnTHCPSi and UnTHCPSi–HA + was evaluated by a CellTiter-Glo ® Luminescent Cell Viability assay, as previously described elsewhere. 26,29 MCF-7 and MDA-MB-231 breast cancer cells were suspended in the corresponding cell culture media at a concentration of 2 × 10 5 cells per mL, and approximately 2 × 10 4 cells per well were seeded in 96-well plates (Corning Inc. Life Sciences, USA). The cells were allowed to attach overnight at 37 °C, after which the cell culture medium was removed and replaced with 100 μL of UnTHCPSi and UnTHCPSi–HA + nanoparticle suspensions at concentrations of 25, 50, and 100 μg mL −1, with 1× HBSS (pH 7.4) and 1% Triton X-100 as positive and negative controls, respectively. After incubating for 6 and 24 h at 37 °C, 100 μL of the assay reagent was added to each well and the number of viable cells was determined by measuring the luminescence from the living cells using a Varioskan Flash fluorometer (Thermo Fisher Scientific Inc., USA). The results presented correspond to the average of at least three independent measurements. Cellular internalization The cellular uptake and intracellular localization of the UnTHCPSi and UnTHCPSi–HA + nanoparticles was assessed by TEM. Approximately 10 5 cells per well of MCF-7 and MDA-MB-231 breast cancer cells were seeded in 24-well plates (Corning Inc. Life Sciences, USA) with each well containing a 13 mm round shaped coverslip and allowed to attach overnight at 37 °C. After removing the cell culture media, 500 μL per well of the nanoparticle suspensions at a concentration of 50 μg mL −1 were added to the wells. After an incubation period of 6 h at 37 °C, the nanoparticle suspensions were carefully removed and the samples were rinsed twice with HBSS–HEPES (pH 7.4). Then, the cells were fixed with 2.5% glutaraldehyde in 0.1 M PBS buffer (pH 7.4) for 1 h at room temperature, and subsequently washed twice with HBSS–HEPES (pH 7.4) and sodium cacodylate buffer (NaCac) for 3 min, prior to post-fixation with 1% osmium tetroxide in 0.1 M NaCac buffer (pH 7.4). Thereafter, the cells were dehydrated with 30–100% ethanol for 10 min and embedded in epoxy resin. Ultrathin sections with approximately 60 nm were sliced parallel to the coverslips, post-stained with uranyl acetate and lead citrate, and finally analyzed by TEM as described above. Flow cytometric analysis of CD44 expression and cellular association The expression of CD44 receptor in MCF-7 and MDA-MB-231 breast cancer cell lines was evaluated by flow cytometry according to a protocol adapted from the manufacturer's instructions, as described in the ESI. †

I’ve always wanted to be a bimbo, in my heart of hearts. I think it’s got something to do with being a kid in the noughties. I grew up on a diet of Playboy bunnies and Pussycat Dolls. And it’s a miracle I didn’t grow up on a diet, full stop. We’re entering the decade of the bimbo, I can feel it in my 300CC breast implants E. C. Wu, J. S. Andrew, L. Cheng, W. R. Freeman, L. Pearson and M. J. Sailor, Biomaterials, 2011, 32, 1957–1966 CrossRef CAS PubMed. N2 - Substitution of fossil fuels by sustainable practices must be rapidly implemented to mitigate the impacts of climate change. The conversion of biomass into combustible gas is investigated in a microwave-induced plasma reactor using pure steam as the plasma working gas for the first time. The optimum results are achieved at the highest forward microwave power of 6 kW with biomass carbon conversion efficiency over 98% and complete biomass energy recovery in syngas. Unreacted steam is simply condensed out, leading to the production of a syngas with low inert dilution and high calorific value in the range 10.5–12 MJ/Nm3. The syngas produced is rich in hydrogen, exceeding 60% by volume. The proposed process could aid in the transition to a carbon neutral economy as it has the potential to efficiently convert biomass to syngas that can be used for the sustainable generation of fuels, chemicals and energy. Kogal or, more correctly, kogyaru and ganguro carry similar connotations as a Japanese version of a "valley girl" or bimbo.

ma bimbo

M. Kovalainen, J. Mönkäre, M. Kaasalainen, J. Riikonen, V.-P. Lehto, J. Salonen, K.-H. Herzig and K. Järvinen, Mol. Pharmaceutics, 2013, 10, 353–359 CrossRef CAS PubMed. If Ma Bimbo first got popular thanks to a big community of female players aged between 20-30, its target demographic has increasingly switched to pre-teen and teen girls. Because of the big age gaps between the players, the staff struggled to satisfy everyone. The older players wanted bolder and sexier clothes while the younger ones wanted Disney-inspired apparel. Ultimately, the developers sided with the younger crowd through the release of Twilight, Hunger Games and Disney rip-off costumes (without the agreement of the companies behind the franchises, by the way). Nineland (who's an adult woman) also changed the way she communicated with the players by infantilizing her language (basically she used 'baby-ish' wordings of common words instead of the normal wordings, for French speakers, she replaced "trop" with "cro" among other stuffs). The last but not least drama: the "auction house" drama. One of the game feature is an auction house where you can buy exclusive clothing through bidding. The more exclusive the clothing is the higher the bids can go. However, because of the high price tags for most items, many players don't participate to the bidding as they would need to buy credits to get more in-game money (or bimbos d'or). If at first, the staff certified that they would never force players to bid, new levels introduced a task: win an auction. The more you advance in the game, the more auctions you're required to win so you can level up. M. Kilpeläinen, J. Mönkäre, M. A. Vlasova, J. Riikonen, V.-P. Lehto, J. Salonen, K. Järvinen and K.-H. Herzig, Eur. J. Pharm. Biopharm., 2011, 77, 20–25 CrossRef PubMed. M. Sarparanta, L. M. Bimbo, J. Rytkönen, E. Mäkilä, T. J. Laaksonen, P. Laaksonen, M. Nyman, J. Salonen, M. B. Linder, J. Hirvonen, H. A. Santos and A. J. Airaksinen, Mol. Pharmaceutics, 2012, 9, 654–663 CrossRef CAS PubMed.